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If we know how much mass is lost, we can use Einstein's equation, E = mc.This relationship shows that even a small change in mass results in a large change in energy.If we add enough energy to a nucleus and split it apart, we will see an increase in mass because this is proportional to the amount of energy we added. If we were to flip that arrow around and take all of our individual particles (with the higher total mass) and fuse them together to form a nucleus, we would get a helium nucleus (with a lower mass) and a lot of energy would be released.
In this situation, however, we need to be more precise.
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What this equation states is that mass and energy are directly proportional to each other.
If a substance gains mass, it will gain energy; if it loses mass, it will lose energy.