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Donor 1 was seropositive for HCMV, Donor 72 was seronegative.PBMC from both donors were tested in an IFN-γ Immuno Spot® assay in medium alone as the negative control (“MEDIUM”), or in the presence of the HCMVpp65 peptide pool (HCMVpp65) or inactivated HCMV virions (I-HCMV), as specified.
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As positive controls for eliciting memory T-cell responses, a pool of 32 peptides of HCMV, EBV, and influenza virus were used (CEFpp).
The test was performed as specified in Materials and Methods, with three replicate wells for each condition.